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map2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc map2
    CSDS upregulates Drp1 expression in hippocampal neurons. A) Differential protein volcano map of CSDS group and Control group (n = 3 samples per group; each sample pooled from 6 hippocampi). Red: significantly upregulated (fold change >1.5); blue: significantly downregulated (fold change >1.5); gray: non-significant. B) CO-IP analysis of Drp1 protein and its ubiquitination level in hippocampus of three groups of mice. One-way ANOVA with Tukey's multiple comparisons test (Treatment, F (2, 15) = 162.8, P < 0.0001; Treatment, F (2, 15) = 12.10, P = 0.0007). Data are expressed as mean ± SEM (n = 6 mice per group). ∗∗ p < 0.01, ∗∗∗ p < 0.001 C) Representative images of Drp1 (green), <t>MAP2</t> (neuron, red) and DAPI (nucleus, blue) in CA1 of two groups (left) and Pearson correlation coefficient was quantitatively analyzed (right, n = 5 mice brain slices). Scale bar: 50 μm. D) Experimental timeline of viral injection and CSDS modeling; Representative confocal Z-stack 3D reconstructed images of dendritic spines in CA1 (left) and dendritic spines was quantitatively analyzed (right, n = 5 mice brain slices). Scale bar: 2 μm. Student's t -test (P = 0.0041; P = 0.0006). Data are expressed as mean ± SEM. ∗∗ p < 0.01, ∗∗∗ p < 0.001. E) Representative images of Drp1 (green), VGLUT2 (Neuron, red) and DAPI (nucleus, blue) in hippocampal CA1 region of two groups (left) and the percentage of co-localized fluorescence was quantified (right). Scale bar: 50 μm. Two-way ANOVA with Tukey's multiple comparisons test (Interaction, F (2, 24) = 2.671, P = 0.0897). The data were expressed as mean ± SEM (n = 5 mice brain slices). ns, p > 0.05.
    Map2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 817 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+map2+antibody/MAP2+Antibody/pmc12996796-121-14-18
    Average 96 stars, based on 817 article reviews
    map2 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Excessive ER-mitochondria coupling: A DRP1-driven mechanism underlying mitochondrial dysfunction and impaired autophagy in stress-induced depression-like behavior in mice"

    Article Title: Excessive ER-mitochondria coupling: A DRP1-driven mechanism underlying mitochondrial dysfunction and impaired autophagy in stress-induced depression-like behavior in mice

    Journal: Redox Biology

    doi: 10.1016/j.redox.2026.104121

    CSDS upregulates Drp1 expression in hippocampal neurons. A) Differential protein volcano map of CSDS group and Control group (n = 3 samples per group; each sample pooled from 6 hippocampi). Red: significantly upregulated (fold change >1.5); blue: significantly downregulated (fold change >1.5); gray: non-significant. B) CO-IP analysis of Drp1 protein and its ubiquitination level in hippocampus of three groups of mice. One-way ANOVA with Tukey's multiple comparisons test (Treatment, F (2, 15) = 162.8, P < 0.0001; Treatment, F (2, 15) = 12.10, P = 0.0007). Data are expressed as mean ± SEM (n = 6 mice per group). ∗∗ p < 0.01, ∗∗∗ p < 0.001 C) Representative images of Drp1 (green), MAP2 (neuron, red) and DAPI (nucleus, blue) in CA1 of two groups (left) and Pearson correlation coefficient was quantitatively analyzed (right, n = 5 mice brain slices). Scale bar: 50 μm. D) Experimental timeline of viral injection and CSDS modeling; Representative confocal Z-stack 3D reconstructed images of dendritic spines in CA1 (left) and dendritic spines was quantitatively analyzed (right, n = 5 mice brain slices). Scale bar: 2 μm. Student's t -test (P = 0.0041; P = 0.0006). Data are expressed as mean ± SEM. ∗∗ p < 0.01, ∗∗∗ p < 0.001. E) Representative images of Drp1 (green), VGLUT2 (Neuron, red) and DAPI (nucleus, blue) in hippocampal CA1 region of two groups (left) and the percentage of co-localized fluorescence was quantified (right). Scale bar: 50 μm. Two-way ANOVA with Tukey's multiple comparisons test (Interaction, F (2, 24) = 2.671, P = 0.0897). The data were expressed as mean ± SEM (n = 5 mice brain slices). ns, p > 0.05.
    Figure Legend Snippet: CSDS upregulates Drp1 expression in hippocampal neurons. A) Differential protein volcano map of CSDS group and Control group (n = 3 samples per group; each sample pooled from 6 hippocampi). Red: significantly upregulated (fold change >1.5); blue: significantly downregulated (fold change >1.5); gray: non-significant. B) CO-IP analysis of Drp1 protein and its ubiquitination level in hippocampus of three groups of mice. One-way ANOVA with Tukey's multiple comparisons test (Treatment, F (2, 15) = 162.8, P < 0.0001; Treatment, F (2, 15) = 12.10, P = 0.0007). Data are expressed as mean ± SEM (n = 6 mice per group). ∗∗ p < 0.01, ∗∗∗ p < 0.001 C) Representative images of Drp1 (green), MAP2 (neuron, red) and DAPI (nucleus, blue) in CA1 of two groups (left) and Pearson correlation coefficient was quantitatively analyzed (right, n = 5 mice brain slices). Scale bar: 50 μm. D) Experimental timeline of viral injection and CSDS modeling; Representative confocal Z-stack 3D reconstructed images of dendritic spines in CA1 (left) and dendritic spines was quantitatively analyzed (right, n = 5 mice brain slices). Scale bar: 2 μm. Student's t -test (P = 0.0041; P = 0.0006). Data are expressed as mean ± SEM. ∗∗ p < 0.01, ∗∗∗ p < 0.001. E) Representative images of Drp1 (green), VGLUT2 (Neuron, red) and DAPI (nucleus, blue) in hippocampal CA1 region of two groups (left) and the percentage of co-localized fluorescence was quantified (right). Scale bar: 50 μm. Two-way ANOVA with Tukey's multiple comparisons test (Interaction, F (2, 24) = 2.671, P = 0.0897). The data were expressed as mean ± SEM (n = 5 mice brain slices). ns, p > 0.05.

    Techniques Used: Expressing, Control, Co-Immunoprecipitation Assay, Ubiquitin Proteomics, Injection, Fluorescence

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    Chromatin Immunoprecipitation:

    Article Title: A microengineered 3D human neurovascular unit model to probe the neuropathogenesis of herpes simplex encephalitis
    Article Snippet: .. Investigators were blinded to group allocation during data collection and analysis. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Novel plant genotypes Seed stocks Authentication Plants In immunofluorescence experiments, the primary antibodies below were used: Anti-VE-cadherin antibody (Proteintech Group, 66804-1-Ig, 1:100), Anti-VE-cadherin antibody (Cell Signaling Technology, 2500S, 1:200), Anti-PECAM-1 antibody (HUABIO group, M1511-8, 1:200), Anti-Claudin-5 (ImmunoWay group, YT0952, 1:200), Anti-S100! antibody (Proteintech Group, 15146-1-AP, 1:200), Anti-S100! antibody (Proteintech Group, 66616-1-Ig, 1:100), Anti-GFAP antibody (Cell Signaling Technology, 3670S, 1:100), Anti-GFAP antibody (HUABIO group, EM140707, 1:200), Anti-Tuj-1 antibody (Cell signaling Technology, 5568S, 1:100), Anti-MAP2 antibody (Cell signaling Technology, 4542S, 1:100), Anti-IBA1 antibody (Abcam, Ab178847, 1:100), Anti-CD11b antibody (Abcam, ab52478, 1:100), Anti-CD11b antibody (Proteintech group, 66519-1-Ig, 1:100), Anti-CD68 antibody (Cell signaling Technology, 76437S; 1:200), Anti-CD19 antibody (Abcam, ab134114, 1:200), Anti-CD3 antibody (Abcam, ab135372, 1:200), Anti-CD45 antibody (Abcam, ab8216, 1:200), Anti-IBA1 (Proteintech Group, 66827-1-Ig, 1:100), Anti-IBA1 (Proteintech group, 10904-1-AP, 1:100), Anti-HSV-1 gG antibody (Santa Cruz, Sc-69804, 1:200), Anti-P62 antibody (Abcam, Ab280086, 1:200), Anti-LC3B antibody (Cell signaling Technology, 3868S, 1:200), Anti-ZO-1 antibody (Proteintech Group, 21773-1- AP, 1:200), Anti-Ki67 antibody (Abcam ab, 243878, 1:200), Anti-A!40 antibody (Bioss, bsm-0106M , 1:200), Anti-A!42 antibody (Cell Signaling Technology, 14974T, 1:200). .. In immunofluorescence experiments, the secondary antibodies bellow were used: Anti-mouse IgG conjugated with Alexa Fluor 488 (Abcam, ab150113, 1:500), Anti-rabbit IgG conjugated with Alexa Fluor 488 (Abcam, ab150077, 1:500), Anti-mouse IgG conjugated with Alexa Fluor 594 (Abcam, ab150116, 1:500) and Anti-rabbit IgG conjugated with Alexa Fluor 594 (Abcam, ab150080, 1:500).

    Flow Cytometry:

    Article Title: A microengineered 3D human neurovascular unit model to probe the neuropathogenesis of herpes simplex encephalitis
    Article Snippet: .. Investigators were blinded to group allocation during data collection and analysis. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Novel plant genotypes Seed stocks Authentication Plants In immunofluorescence experiments, the primary antibodies below were used: Anti-VE-cadherin antibody (Proteintech Group, 66804-1-Ig, 1:100), Anti-VE-cadherin antibody (Cell Signaling Technology, 2500S, 1:200), Anti-PECAM-1 antibody (HUABIO group, M1511-8, 1:200), Anti-Claudin-5 (ImmunoWay group, YT0952, 1:200), Anti-S100! antibody (Proteintech Group, 15146-1-AP, 1:200), Anti-S100! antibody (Proteintech Group, 66616-1-Ig, 1:100), Anti-GFAP antibody (Cell Signaling Technology, 3670S, 1:100), Anti-GFAP antibody (HUABIO group, EM140707, 1:200), Anti-Tuj-1 antibody (Cell signaling Technology, 5568S, 1:100), Anti-MAP2 antibody (Cell signaling Technology, 4542S, 1:100), Anti-IBA1 antibody (Abcam, Ab178847, 1:100), Anti-CD11b antibody (Abcam, ab52478, 1:100), Anti-CD11b antibody (Proteintech group, 66519-1-Ig, 1:100), Anti-CD68 antibody (Cell signaling Technology, 76437S; 1:200), Anti-CD19 antibody (Abcam, ab134114, 1:200), Anti-CD3 antibody (Abcam, ab135372, 1:200), Anti-CD45 antibody (Abcam, ab8216, 1:200), Anti-IBA1 (Proteintech Group, 66827-1-Ig, 1:100), Anti-IBA1 (Proteintech group, 10904-1-AP, 1:100), Anti-HSV-1 gG antibody (Santa Cruz, Sc-69804, 1:200), Anti-P62 antibody (Abcam, Ab280086, 1:200), Anti-LC3B antibody (Cell signaling Technology, 3868S, 1:200), Anti-ZO-1 antibody (Proteintech Group, 21773-1- AP, 1:200), Anti-Ki67 antibody (Abcam ab, 243878, 1:200), Anti-A!40 antibody (Bioss, bsm-0106M , 1:200), Anti-A!42 antibody (Cell Signaling Technology, 14974T, 1:200). .. In immunofluorescence experiments, the secondary antibodies bellow were used: Anti-mouse IgG conjugated with Alexa Fluor 488 (Abcam, ab150113, 1:500), Anti-rabbit IgG conjugated with Alexa Fluor 488 (Abcam, ab150077, 1:500), Anti-mouse IgG conjugated with Alexa Fluor 594 (Abcam, ab150116, 1:500) and Anti-rabbit IgG conjugated with Alexa Fluor 594 (Abcam, ab150080, 1:500).

    Magnetic Resonance Imaging:

    Article Title: A microengineered 3D human neurovascular unit model to probe the neuropathogenesis of herpes simplex encephalitis
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    Biomarker Discovery:

    Article Title: A microengineered 3D human neurovascular unit model to probe the neuropathogenesis of herpes simplex encephalitis
    Article Snippet: .. Investigators were blinded to group allocation during data collection and analysis. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Novel plant genotypes Seed stocks Authentication Plants In immunofluorescence experiments, the primary antibodies below were used: Anti-VE-cadherin antibody (Proteintech Group, 66804-1-Ig, 1:100), Anti-VE-cadherin antibody (Cell Signaling Technology, 2500S, 1:200), Anti-PECAM-1 antibody (HUABIO group, M1511-8, 1:200), Anti-Claudin-5 (ImmunoWay group, YT0952, 1:200), Anti-S100! antibody (Proteintech Group, 15146-1-AP, 1:200), Anti-S100! antibody (Proteintech Group, 66616-1-Ig, 1:100), Anti-GFAP antibody (Cell Signaling Technology, 3670S, 1:100), Anti-GFAP antibody (HUABIO group, EM140707, 1:200), Anti-Tuj-1 antibody (Cell signaling Technology, 5568S, 1:100), Anti-MAP2 antibody (Cell signaling Technology, 4542S, 1:100), Anti-IBA1 antibody (Abcam, Ab178847, 1:100), Anti-CD11b antibody (Abcam, ab52478, 1:100), Anti-CD11b antibody (Proteintech group, 66519-1-Ig, 1:100), Anti-CD68 antibody (Cell signaling Technology, 76437S; 1:200), Anti-CD19 antibody (Abcam, ab134114, 1:200), Anti-CD3 antibody (Abcam, ab135372, 1:200), Anti-CD45 antibody (Abcam, ab8216, 1:200), Anti-IBA1 (Proteintech Group, 66827-1-Ig, 1:100), Anti-IBA1 (Proteintech group, 10904-1-AP, 1:100), Anti-HSV-1 gG antibody (Santa Cruz, Sc-69804, 1:200), Anti-P62 antibody (Abcam, Ab280086, 1:200), Anti-LC3B antibody (Cell signaling Technology, 3868S, 1:200), Anti-ZO-1 antibody (Proteintech Group, 21773-1- AP, 1:200), Anti-Ki67 antibody (Abcam ab, 243878, 1:200), Anti-A!40 antibody (Bioss, bsm-0106M , 1:200), Anti-A!42 antibody (Cell Signaling Technology, 14974T, 1:200). .. In immunofluorescence experiments, the secondary antibodies bellow were used: Anti-mouse IgG conjugated with Alexa Fluor 488 (Abcam, ab150113, 1:500), Anti-rabbit IgG conjugated with Alexa Fluor 488 (Abcam, ab150077, 1:500), Anti-mouse IgG conjugated with Alexa Fluor 594 (Abcam, ab150116, 1:500) and Anti-rabbit IgG conjugated with Alexa Fluor 594 (Abcam, ab150080, 1:500).

    Immunofluorescence:

    Article Title: A microengineered 3D human neurovascular unit model to probe the neuropathogenesis of herpes simplex encephalitis
    Article Snippet: .. Investigators were blinded to group allocation during data collection and analysis. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Novel plant genotypes Seed stocks Authentication Plants In immunofluorescence experiments, the primary antibodies below were used: Anti-VE-cadherin antibody (Proteintech Group, 66804-1-Ig, 1:100), Anti-VE-cadherin antibody (Cell Signaling Technology, 2500S, 1:200), Anti-PECAM-1 antibody (HUABIO group, M1511-8, 1:200), Anti-Claudin-5 (ImmunoWay group, YT0952, 1:200), Anti-S100! antibody (Proteintech Group, 15146-1-AP, 1:200), Anti-S100! antibody (Proteintech Group, 66616-1-Ig, 1:100), Anti-GFAP antibody (Cell Signaling Technology, 3670S, 1:100), Anti-GFAP antibody (HUABIO group, EM140707, 1:200), Anti-Tuj-1 antibody (Cell signaling Technology, 5568S, 1:100), Anti-MAP2 antibody (Cell signaling Technology, 4542S, 1:100), Anti-IBA1 antibody (Abcam, Ab178847, 1:100), Anti-CD11b antibody (Abcam, ab52478, 1:100), Anti-CD11b antibody (Proteintech group, 66519-1-Ig, 1:100), Anti-CD68 antibody (Cell signaling Technology, 76437S; 1:200), Anti-CD19 antibody (Abcam, ab134114, 1:200), Anti-CD3 antibody (Abcam, ab135372, 1:200), Anti-CD45 antibody (Abcam, ab8216, 1:200), Anti-IBA1 (Proteintech Group, 66827-1-Ig, 1:100), Anti-IBA1 (Proteintech group, 10904-1-AP, 1:100), Anti-HSV-1 gG antibody (Santa Cruz, Sc-69804, 1:200), Anti-P62 antibody (Abcam, Ab280086, 1:200), Anti-LC3B antibody (Cell signaling Technology, 3868S, 1:200), Anti-ZO-1 antibody (Proteintech Group, 21773-1- AP, 1:200), Anti-Ki67 antibody (Abcam ab, 243878, 1:200), Anti-A!40 antibody (Bioss, bsm-0106M , 1:200), Anti-A!42 antibody (Cell Signaling Technology, 14974T, 1:200). .. In immunofluorescence experiments, the secondary antibodies bellow were used: Anti-mouse IgG conjugated with Alexa Fluor 488 (Abcam, ab150113, 1:500), Anti-rabbit IgG conjugated with Alexa Fluor 488 (Abcam, ab150077, 1:500), Anti-mouse IgG conjugated with Alexa Fluor 594 (Abcam, ab150116, 1:500) and Anti-rabbit IgG conjugated with Alexa Fluor 594 (Abcam, ab150080, 1:500).



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    Fitc Conjugated Rabbit Anti Mouse Map2 Primary Antibody, supplied by Wuhan Fine Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+map2+antibody/anti+conjugated+goat+hrp+igg+rabbit/pm42056801-140-1-7
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    nb300 213  (Novus Biologicals)
    96
    Novus Biologicals nb300 213
    CSDS upregulates Drp1 expression in hippocampal neurons. A) Differential protein volcano map of CSDS group and Control group (n = 3 samples per group; each sample pooled from 6 hippocampi). Red: significantly upregulated (fold change >1.5); blue: significantly downregulated (fold change >1.5); gray: non-significant. B) CO-IP analysis of Drp1 protein and its ubiquitination level in hippocampus of three groups of mice. One-way ANOVA with Tukey's multiple comparisons test (Treatment, F (2, 15) = 162.8, P < 0.0001; Treatment, F (2, 15) = 12.10, P = 0.0007). Data are expressed as mean ± SEM (n = 6 mice per group). ∗∗ p < 0.01, ∗∗∗ p < 0.001 C) Representative images of Drp1 (green), <t>MAP2</t> (neuron, red) and DAPI (nucleus, blue) in CA1 of two groups (left) and Pearson correlation coefficient was quantitatively analyzed (right, n = 5 mice brain slices). Scale bar: 50 μm. D) Experimental timeline of viral injection and CSDS modeling; Representative confocal Z-stack 3D reconstructed images of dendritic spines in CA1 (left) and dendritic spines was quantitatively analyzed (right, n = 5 mice brain slices). Scale bar: 2 μm. Student's t -test (P = 0.0041; P = 0.0006). Data are expressed as mean ± SEM. ∗∗ p < 0.01, ∗∗∗ p < 0.001. E) Representative images of Drp1 (green), VGLUT2 (Neuron, red) and DAPI (nucleus, blue) in hippocampal CA1 region of two groups (left) and the percentage of co-localized fluorescence was quantified (right). Scale bar: 50 μm. Two-way ANOVA with Tukey's multiple comparisons test (Interaction, F (2, 24) = 2.671, P = 0.0897). The data were expressed as mean ± SEM (n = 5 mice brain slices). ns, p > 0.05.
    Nb300 213, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    map2  (Novus Biologicals)
    96
    Novus Biologicals map2
    Protein level differences in senescence-associated markers further distinguish neuronal and fibroblast responses to DNA damage (A) Schematic of immunocytochemistry (ICC) performed after IR in healthy dermal fibroblasts and iNs from the same donors (donors: A27, A48, ID98; 3 technical replicates per donor in each condition). 0.2% FBS low serum (LS) treatment was applied to CTL and IR fibroblasts to induce quiescence 72 hours prior to collection. (B) IR iNs and IR fibroblasts were stained for p21 (top left), p16 (top right), LMNB1 (bottom left), and HMGB1 (bottom right). Mean nuclear fluorescence intensity was quantified. (C) Nuclear area of IR iNs and IR fibroblasts quantified by DAPI staining (donors: A27, A48, ID98, 18 technical replicates per donor in each condition). (D) PSD95 staining for IR iNs and IR fibroblasts, imaged by confocal microscopy, and analyzed by Imaris to detect PSD95 spots on <t>MAP2-positive</t> dendrites and normalize to dendrite surface area. Two donors used for PSD95 staining (donors: A27, A48; 3 technical replicates per donor in each condition; donor A48 images and quantification shown in ). Tracks on MAP2 traced in Illustrator. All iNs imaged were quantified with automatic MAP2 tracing to measure p21, p16, LMNB1, HMGB1, and DAPI only in neuronal nuclei or PSD95 only in neuronal dendrites (Supp. Fig. 5). Imaging was performed on the Harmony Operetta CLS system at 63X unless otherwise stated. Nuclear intensity quantification of images performed by the Harmony analysis system and nuclei number after IR provided in Supp. Fig. 4. Statistics were calculated using multiple unpaired t-tests using Welch’s correction with Holm–Šídák adjustment for multiple testing with alpha = 0.05. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not significant, nd: not detected.
    Map2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti map2  (Fisher Scientific)
    86
    Fisher Scientific anti map2
    Protein level differences in senescence-associated markers further distinguish neuronal and fibroblast responses to DNA damage (A) Schematic of immunocytochemistry (ICC) performed after IR in healthy dermal fibroblasts and iNs from the same donors (donors: A27, A48, ID98; 3 technical replicates per donor in each condition). 0.2% FBS low serum (LS) treatment was applied to CTL and IR fibroblasts to induce quiescence 72 hours prior to collection. (B) IR iNs and IR fibroblasts were stained for p21 (top left), p16 (top right), LMNB1 (bottom left), and HMGB1 (bottom right). Mean nuclear fluorescence intensity was quantified. (C) Nuclear area of IR iNs and IR fibroblasts quantified by DAPI staining (donors: A27, A48, ID98, 18 technical replicates per donor in each condition). (D) PSD95 staining for IR iNs and IR fibroblasts, imaged by confocal microscopy, and analyzed by Imaris to detect PSD95 spots on <t>MAP2-positive</t> dendrites and normalize to dendrite surface area. Two donors used for PSD95 staining (donors: A27, A48; 3 technical replicates per donor in each condition; donor A48 images and quantification shown in ). Tracks on MAP2 traced in Illustrator. All iNs imaged were quantified with automatic MAP2 tracing to measure p21, p16, LMNB1, HMGB1, and DAPI only in neuronal nuclei or PSD95 only in neuronal dendrites (Supp. Fig. 5). Imaging was performed on the Harmony Operetta CLS system at 63X unless otherwise stated. Nuclear intensity quantification of images performed by the Harmony analysis system and nuclei number after IR provided in Supp. Fig. 4. Statistics were calculated using multiple unpaired t-tests using Welch’s correction with Holm–Šídák adjustment for multiple testing with alpha = 0.05. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not significant, nd: not detected.
    Anti Map2, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti map2  (Novus Biologicals)
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    Protein level differences in senescence-associated markers further distinguish neuronal and fibroblast responses to DNA damage (A) Schematic of immunocytochemistry (ICC) performed after IR in healthy dermal fibroblasts and iNs from the same donors (donors: A27, A48, ID98; 3 technical replicates per donor in each condition). 0.2% FBS low serum (LS) treatment was applied to CTL and IR fibroblasts to induce quiescence 72 hours prior to collection. (B) IR iNs and IR fibroblasts were stained for p21 (top left), p16 (top right), LMNB1 (bottom left), and HMGB1 (bottom right). Mean nuclear fluorescence intensity was quantified. (C) Nuclear area of IR iNs and IR fibroblasts quantified by DAPI staining (donors: A27, A48, ID98, 18 technical replicates per donor in each condition). (D) PSD95 staining for IR iNs and IR fibroblasts, imaged by confocal microscopy, and analyzed by Imaris to detect PSD95 spots on <t>MAP2-positive</t> dendrites and normalize to dendrite surface area. Two donors used for PSD95 staining (donors: A27, A48; 3 technical replicates per donor in each condition; donor A48 images and quantification shown in ). Tracks on MAP2 traced in Illustrator. All iNs imaged were quantified with automatic MAP2 tracing to measure p21, p16, LMNB1, HMGB1, and DAPI only in neuronal nuclei or PSD95 only in neuronal dendrites (Supp. Fig. 5). Imaging was performed on the Harmony Operetta CLS system at 63X unless otherwise stated. Nuclear intensity quantification of images performed by the Harmony analysis system and nuclei number after IR provided in Supp. Fig. 4. Statistics were calculated using multiple unpaired t-tests using Welch’s correction with Holm–Šídák adjustment for multiple testing with alpha = 0.05. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not significant, nd: not detected.
    Rabbit Anti Map2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti map2 polyclonal  (Proteintech)
    96
    Proteintech rabbit anti map2 polyclonal
    Protein level differences in senescence-associated markers further distinguish neuronal and fibroblast responses to DNA damage (A) Schematic of immunocytochemistry (ICC) performed after IR in healthy dermal fibroblasts and iNs from the same donors (donors: A27, A48, ID98; 3 technical replicates per donor in each condition). 0.2% FBS low serum (LS) treatment was applied to CTL and IR fibroblasts to induce quiescence 72 hours prior to collection. (B) IR iNs and IR fibroblasts were stained for p21 (top left), p16 (top right), LMNB1 (bottom left), and HMGB1 (bottom right). Mean nuclear fluorescence intensity was quantified. (C) Nuclear area of IR iNs and IR fibroblasts quantified by DAPI staining (donors: A27, A48, ID98, 18 technical replicates per donor in each condition). (D) PSD95 staining for IR iNs and IR fibroblasts, imaged by confocal microscopy, and analyzed by Imaris to detect PSD95 spots on <t>MAP2-positive</t> dendrites and normalize to dendrite surface area. Two donors used for PSD95 staining (donors: A27, A48; 3 technical replicates per donor in each condition; donor A48 images and quantification shown in ). Tracks on MAP2 traced in Illustrator. All iNs imaged were quantified with automatic MAP2 tracing to measure p21, p16, LMNB1, HMGB1, and DAPI only in neuronal nuclei or PSD95 only in neuronal dendrites (Supp. Fig. 5). Imaging was performed on the Harmony Operetta CLS system at 63X unless otherwise stated. Nuclear intensity quantification of images performed by the Harmony analysis system and nuclei number after IR provided in Supp. Fig. 4. Statistics were calculated using multiple unpaired t-tests using Welch’s correction with Holm–Šídák adjustment for multiple testing with alpha = 0.05. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not significant, nd: not detected.
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    Image Search Results


    A) HE pathology staining, MAP2 immunohistochemistry, and silver staining of brain tissue from various groups of mice. The right side of panel A shows a schematic of the observed brain damage areas (Image credit: Allen Institute for Brain Science), with black dots indicating the damaged areas, primarily in the hippocampal region. B) Statistical count of infiltrated immune cells and microvessels affected by thromboembolic events in each group of mice. * p < 0.05, ** p < 0.01.

    Journal: PLOS One

    Article Title: Inhibition of neutrophil infiltration and NETs formation ameliorates neuropsychiatric and renal dysfunction in MRL/lpr mice with lupus

    doi: 10.1371/journal.pone.0348011

    Figure Lengend Snippet: A) HE pathology staining, MAP2 immunohistochemistry, and silver staining of brain tissue from various groups of mice. The right side of panel A shows a schematic of the observed brain damage areas (Image credit: Allen Institute for Brain Science), with black dots indicating the damaged areas, primarily in the hippocampal region. B) Statistical count of infiltrated immune cells and microvessels affected by thromboembolic events in each group of mice. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies were applied overnight at 4°C, including: MAP2 (1:2000, GB11128-2, Servicebio).

    Techniques: Staining, Immunohistochemistry, Silver Staining

    A) Upper half: Multiplex immunofluorescence staining of CD11b, CD16, and MAP2 in brain sections from various groups of mice, with localized positive areas enlarged; white arrowheads indicate CD11b/CD16 double-positive cells. Lower half: Multiplex immunofluorescence staining of NE and MPO, with localized positive areas enlarged. The brain region schematic used as a reference is derived from the Allen Brain Atlas. B) and C) Statistical count of CD11b positive and CD11b/CD16 double-positive cells across different groups. D) and E) Semi-quantitative analysis of fluorescence intensity for NE and MPO in different groups. Statistical significance is indicated as follows: * p < 0.05, ** p < 0.01 compared to the MRL/MpJ group; # p < 0.05, ## p < 0.01 compared to the MRL/lpr+Avacopan group.

    Journal: PLOS One

    Article Title: Inhibition of neutrophil infiltration and NETs formation ameliorates neuropsychiatric and renal dysfunction in MRL/lpr mice with lupus

    doi: 10.1371/journal.pone.0348011

    Figure Lengend Snippet: A) Upper half: Multiplex immunofluorescence staining of CD11b, CD16, and MAP2 in brain sections from various groups of mice, with localized positive areas enlarged; white arrowheads indicate CD11b/CD16 double-positive cells. Lower half: Multiplex immunofluorescence staining of NE and MPO, with localized positive areas enlarged. The brain region schematic used as a reference is derived from the Allen Brain Atlas. B) and C) Statistical count of CD11b positive and CD11b/CD16 double-positive cells across different groups. D) and E) Semi-quantitative analysis of fluorescence intensity for NE and MPO in different groups. Statistical significance is indicated as follows: * p < 0.05, ** p < 0.01 compared to the MRL/MpJ group; # p < 0.05, ## p < 0.01 compared to the MRL/lpr+Avacopan group.

    Article Snippet: Primary antibodies were applied overnight at 4°C, including: MAP2 (1:2000, GB11128-2, Servicebio).

    Techniques: Multiplex Assay, Immunofluorescence, Staining, Derivative Assay, Fluorescence

    CSDS upregulates Drp1 expression in hippocampal neurons. A) Differential protein volcano map of CSDS group and Control group (n = 3 samples per group; each sample pooled from 6 hippocampi). Red: significantly upregulated (fold change >1.5); blue: significantly downregulated (fold change >1.5); gray: non-significant. B) CO-IP analysis of Drp1 protein and its ubiquitination level in hippocampus of three groups of mice. One-way ANOVA with Tukey's multiple comparisons test (Treatment, F (2, 15) = 162.8, P < 0.0001; Treatment, F (2, 15) = 12.10, P = 0.0007). Data are expressed as mean ± SEM (n = 6 mice per group). ∗∗ p < 0.01, ∗∗∗ p < 0.001 C) Representative images of Drp1 (green), MAP2 (neuron, red) and DAPI (nucleus, blue) in CA1 of two groups (left) and Pearson correlation coefficient was quantitatively analyzed (right, n = 5 mice brain slices). Scale bar: 50 μm. D) Experimental timeline of viral injection and CSDS modeling; Representative confocal Z-stack 3D reconstructed images of dendritic spines in CA1 (left) and dendritic spines was quantitatively analyzed (right, n = 5 mice brain slices). Scale bar: 2 μm. Student's t -test (P = 0.0041; P = 0.0006). Data are expressed as mean ± SEM. ∗∗ p < 0.01, ∗∗∗ p < 0.001. E) Representative images of Drp1 (green), VGLUT2 (Neuron, red) and DAPI (nucleus, blue) in hippocampal CA1 region of two groups (left) and the percentage of co-localized fluorescence was quantified (right). Scale bar: 50 μm. Two-way ANOVA with Tukey's multiple comparisons test (Interaction, F (2, 24) = 2.671, P = 0.0897). The data were expressed as mean ± SEM (n = 5 mice brain slices). ns, p > 0.05.

    Journal: Redox Biology

    Article Title: Excessive ER-mitochondria coupling: A DRP1-driven mechanism underlying mitochondrial dysfunction and impaired autophagy in stress-induced depression-like behavior in mice

    doi: 10.1016/j.redox.2026.104121

    Figure Lengend Snippet: CSDS upregulates Drp1 expression in hippocampal neurons. A) Differential protein volcano map of CSDS group and Control group (n = 3 samples per group; each sample pooled from 6 hippocampi). Red: significantly upregulated (fold change >1.5); blue: significantly downregulated (fold change >1.5); gray: non-significant. B) CO-IP analysis of Drp1 protein and its ubiquitination level in hippocampus of three groups of mice. One-way ANOVA with Tukey's multiple comparisons test (Treatment, F (2, 15) = 162.8, P < 0.0001; Treatment, F (2, 15) = 12.10, P = 0.0007). Data are expressed as mean ± SEM (n = 6 mice per group). ∗∗ p < 0.01, ∗∗∗ p < 0.001 C) Representative images of Drp1 (green), MAP2 (neuron, red) and DAPI (nucleus, blue) in CA1 of two groups (left) and Pearson correlation coefficient was quantitatively analyzed (right, n = 5 mice brain slices). Scale bar: 50 μm. D) Experimental timeline of viral injection and CSDS modeling; Representative confocal Z-stack 3D reconstructed images of dendritic spines in CA1 (left) and dendritic spines was quantitatively analyzed (right, n = 5 mice brain slices). Scale bar: 2 μm. Student's t -test (P = 0.0041; P = 0.0006). Data are expressed as mean ± SEM. ∗∗ p < 0.01, ∗∗∗ p < 0.001. E) Representative images of Drp1 (green), VGLUT2 (Neuron, red) and DAPI (nucleus, blue) in hippocampal CA1 region of two groups (left) and the percentage of co-localized fluorescence was quantified (right). Scale bar: 50 μm. Two-way ANOVA with Tukey's multiple comparisons test (Interaction, F (2, 24) = 2.671, P = 0.0897). The data were expressed as mean ± SEM (n = 5 mice brain slices). ns, p > 0.05.

    Article Snippet: Multiplex staining was performed using TSA-based immunofluorescence with primary antibodies: Drp1 (CST 12741S, 1:100), MAP2 (ab32454, 1:1000), GFAP (CST 12389, 1:400), IBA1 (ab178846, 1:1000), Vglut1 (CST #27181, 1:800), Vglut2 (ab216463, 1:250), OLIG2 (CST #65915, 1:400), and LAMP1 (CST 99437S, 1:100).

    Techniques: Expressing, Control, Co-Immunoprecipitation Assay, Ubiquitin Proteomics, Injection, Fluorescence

    Protein level differences in senescence-associated markers further distinguish neuronal and fibroblast responses to DNA damage (A) Schematic of immunocytochemistry (ICC) performed after IR in healthy dermal fibroblasts and iNs from the same donors (donors: A27, A48, ID98; 3 technical replicates per donor in each condition). 0.2% FBS low serum (LS) treatment was applied to CTL and IR fibroblasts to induce quiescence 72 hours prior to collection. (B) IR iNs and IR fibroblasts were stained for p21 (top left), p16 (top right), LMNB1 (bottom left), and HMGB1 (bottom right). Mean nuclear fluorescence intensity was quantified. (C) Nuclear area of IR iNs and IR fibroblasts quantified by DAPI staining (donors: A27, A48, ID98, 18 technical replicates per donor in each condition). (D) PSD95 staining for IR iNs and IR fibroblasts, imaged by confocal microscopy, and analyzed by Imaris to detect PSD95 spots on MAP2-positive dendrites and normalize to dendrite surface area. Two donors used for PSD95 staining (donors: A27, A48; 3 technical replicates per donor in each condition; donor A48 images and quantification shown in ). Tracks on MAP2 traced in Illustrator. All iNs imaged were quantified with automatic MAP2 tracing to measure p21, p16, LMNB1, HMGB1, and DAPI only in neuronal nuclei or PSD95 only in neuronal dendrites (Supp. Fig. 5). Imaging was performed on the Harmony Operetta CLS system at 63X unless otherwise stated. Nuclear intensity quantification of images performed by the Harmony analysis system and nuclei number after IR provided in Supp. Fig. 4. Statistics were calculated using multiple unpaired t-tests using Welch’s correction with Holm–Šídák adjustment for multiple testing with alpha = 0.05. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not significant, nd: not detected.

    Journal: bioRxiv

    Article Title: DNA damage drives a unique, Alzheimer’s disease-relevant senescent state in neurons

    doi: 10.64898/2026.04.02.716205

    Figure Lengend Snippet: Protein level differences in senescence-associated markers further distinguish neuronal and fibroblast responses to DNA damage (A) Schematic of immunocytochemistry (ICC) performed after IR in healthy dermal fibroblasts and iNs from the same donors (donors: A27, A48, ID98; 3 technical replicates per donor in each condition). 0.2% FBS low serum (LS) treatment was applied to CTL and IR fibroblasts to induce quiescence 72 hours prior to collection. (B) IR iNs and IR fibroblasts were stained for p21 (top left), p16 (top right), LMNB1 (bottom left), and HMGB1 (bottom right). Mean nuclear fluorescence intensity was quantified. (C) Nuclear area of IR iNs and IR fibroblasts quantified by DAPI staining (donors: A27, A48, ID98, 18 technical replicates per donor in each condition). (D) PSD95 staining for IR iNs and IR fibroblasts, imaged by confocal microscopy, and analyzed by Imaris to detect PSD95 spots on MAP2-positive dendrites and normalize to dendrite surface area. Two donors used for PSD95 staining (donors: A27, A48; 3 technical replicates per donor in each condition; donor A48 images and quantification shown in ). Tracks on MAP2 traced in Illustrator. All iNs imaged were quantified with automatic MAP2 tracing to measure p21, p16, LMNB1, HMGB1, and DAPI only in neuronal nuclei or PSD95 only in neuronal dendrites (Supp. Fig. 5). Imaging was performed on the Harmony Operetta CLS system at 63X unless otherwise stated. Nuclear intensity quantification of images performed by the Harmony analysis system and nuclei number after IR provided in Supp. Fig. 4. Statistics were calculated using multiple unpaired t-tests using Welch’s correction with Holm–Šídák adjustment for multiple testing with alpha = 0.05. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not significant, nd: not detected.

    Article Snippet: Cells were then incubated with primary antibodies at 4°C overnight (γH2AX, abcam, ab81299, 1:250; 1:1000; pATM, Life Technologies, MA1-2020, 1:250; p21, abcam, ab109520, 1:100; p16, abcam, ab270058, 1:50; LMNB1, abcam, ab229025, 1:1000; HMGB1, abcam, ab79823, 1:250; 53BP1, abcam, ab175933, 1:200, PSD95, Life Technologies, MA1-046, 1:300; MAP2, Novus Biologicals, NB300-213, 1:400).

    Techniques: Immunocytochemistry, Staining, Fluorescence, Confocal Microscopy, Imaging

    DNA damage induces greater damage lesions and a slower DNA repair-associated response in neurons compared to fibroblasts (A) Schematic of immunocytochemistry (ICC) performed over time after IR in healthy dermal fibroblasts and iNs from the same donors (donors: A48, ID97; 3 technical replicates per donor in each condition). (B-D) iNs and fibroblasts were stained for 53BP1 (B), γH2AX (C), and pATM (D) at 0 min, 15 min, 2h, and 24h after IR with magnified images from the viewfield in the bottom right of each image. Relative fold change (right panels) was calculated from mean foci number per nucleus (middle panels). All iNs imaged were quantified with automatic MAP2 tracing to measure 53BP1, γH2AX, and pATM foci only in neuronal nuclei (Supp. Fig. 5). Imaging was performed on the Harmony Operetta CLS system at 63X. Foci quantification of images performed by the Harmony analysis system. Intensity over time without relative fold change calculations provided in Supp. Fig. 6 along with nuclei number over time after IR. Statistics were calculated by two-way ANOVA (or Mixed Model) and corrected for multiple comparisons using the Dunnett method and reporting multiplicity-adjusted p-value for each comparison with alpha = 0.05. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not significant, nd: not detected.

    Journal: bioRxiv

    Article Title: DNA damage drives a unique, Alzheimer’s disease-relevant senescent state in neurons

    doi: 10.64898/2026.04.02.716205

    Figure Lengend Snippet: DNA damage induces greater damage lesions and a slower DNA repair-associated response in neurons compared to fibroblasts (A) Schematic of immunocytochemistry (ICC) performed over time after IR in healthy dermal fibroblasts and iNs from the same donors (donors: A48, ID97; 3 technical replicates per donor in each condition). (B-D) iNs and fibroblasts were stained for 53BP1 (B), γH2AX (C), and pATM (D) at 0 min, 15 min, 2h, and 24h after IR with magnified images from the viewfield in the bottom right of each image. Relative fold change (right panels) was calculated from mean foci number per nucleus (middle panels). All iNs imaged were quantified with automatic MAP2 tracing to measure 53BP1, γH2AX, and pATM foci only in neuronal nuclei (Supp. Fig. 5). Imaging was performed on the Harmony Operetta CLS system at 63X. Foci quantification of images performed by the Harmony analysis system. Intensity over time without relative fold change calculations provided in Supp. Fig. 6 along with nuclei number over time after IR. Statistics were calculated by two-way ANOVA (or Mixed Model) and corrected for multiple comparisons using the Dunnett method and reporting multiplicity-adjusted p-value for each comparison with alpha = 0.05. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not significant, nd: not detected.

    Article Snippet: Cells were then incubated with primary antibodies at 4°C overnight (γH2AX, abcam, ab81299, 1:250; 1:1000; pATM, Life Technologies, MA1-2020, 1:250; p21, abcam, ab109520, 1:100; p16, abcam, ab270058, 1:50; LMNB1, abcam, ab229025, 1:1000; HMGB1, abcam, ab79823, 1:250; 53BP1, abcam, ab175933, 1:200, PSD95, Life Technologies, MA1-046, 1:300; MAP2, Novus Biologicals, NB300-213, 1:400).

    Techniques: Immunocytochemistry, Staining, Imaging, Comparison